MRNA expression profiling of hepatocellular carcinoma induced by AAV in vivo gene targeting at the Rian locus

ABSTRACT: The distinct phenotypic and prognostic subclasses of human hepatocellular carcinoma (HCC) are difficult to reproduce in animal experiments. Here we have used in vivo gene targeting to insert an enhancer-promoter element at an imprinted chromosome 12 locus in mice, thereby converting ~1 in 20,000 normal hepatocytes into a focus of HCC with a single genetic modification. A 300 kb chromosomal domain containing multiple mRNAs, snoRNAs and microRNAs was activated surrounding the integration site. An identical domain was activated at the syntenic locus in a specific molecular subclass of spontaneous human HCCs with a similar histological phenotype, which was associated with partial loss of DNA methylation. These findings demonstrate the accuracy of in vivo gene targeting in modeling human

ORGANISM(S): Mus musculus

SUBMITTER: Mei Xu 

PROVIDER: E-GEOD-35403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress