Gene expression profiling of in vitro derived induced and natural FOXP3+ regulatory T cells and ex-iTreg cells in the mouse

ABSTRACT: Induced Treg (iTreg) cells are essential for tolerance and can be used therapeutically, yet their stability in vivo and mechanisms of suppression are unresolved. Here, we used a treatment model of colitis to examine the role of autologous IL-10 in iTreg cell function. Mice treated with IL-10+/+ iTreg cells in combination with IL-10–/– natural Treg (nTreg) cells survived and gained weight, even though iTreg cells were numerically disadvantaged and comprised just ~20% of all Treg cells in treated mice. Notably, ~85% of the transferred iTreg cells lost Foxp3 expression (ex-iTreg) but retained a portion of the iTreg transcriptome which failed to limit their pathogenic potential. The TCR repertoires of iTreg and ex-iTreg cells exhibited almost no overlap, which indicates that the two populat

ORGANISM(S): Mus musculus

SUBMITTER: Martin Hessner 

PROVIDER: E-GEOD-35543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress