LS001A: Aging/Early Degeneration Of The Intervertebral Disc Involves Downregulation Of Canonical Wnt Signaling and Caveolin-1 expression

ABSTRACT: Objective: Aging and early degeneration of the intervertebral disc (IVD) involves the substition of notochordal cells (NCs) in the nucleus pulposus (NP) by chondrocyte-like cells (CLCs). This study investigated the gene expression profiles involved in this process using NP tissue from both non-chondrodystrophic and chondrodystrophic dogs, a species with naturally occuring IVD degeneration. Methods: Dual channel DNA microarrays were used to compare 1) healthy NP tissue, 2) NP tissue with a mixed population of NCs and CLCs, and 3) NP tissue containing solely CLCs. Canonical Wnt-signaling was validated using qPCR of relevant Wnt target genes. Caveolin-1, a known regulator of canonical Wnt signaling, was investigated further in tissue sections using qPCR and immunohistochemistry, and in cultur

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-GEOD-35717 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress