The PARN deadenylase targets a discrete set of mRNAs for decay and regulates cell motility in mouse myoblasts

ABSTRACT: Almost all cellular mRNAs terminate in a 3M-bM-^@M-^Y poly(A) tail, the removal of which can induce both translational silencing and mRNA decay. Mammalian cells encode many poly(A)-specific exoribonucleases but their individual roles are poorly understood. Here, we undertook an analysis of the role of PARN deadenylase in mouse myoblasts using global measurements of mRNA decay rates. Our results reveal that a discrete set of mRNAs exhibit altered mRNA decay as a result of PARN depletion and that stabilization is associated with increased poly(A) tail length and translation. We determined that stabilization of mRNAs does not generally result in their increased abundance supporting the idea that mRNA decay is coupled to transcription. Importantly, PARN knockdown has wide ranging effects

ORGANISM(S): Mus musculus

SUBMITTER: Ju Youn Lee 

PROVIDER: E-GEOD-35944 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress