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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The role of the Tra1p transcription factor of Magnaporthe oryzae in spore adhesion and pathogenic development

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE36224: Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain GSE36225: Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain Refer to individual Series

ORGANISM(S): Magnaporthe oryzae

SUBMITTER: Karsten Andresen

PROVIDER: E-GEOD-36226 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain

Project description:In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in aerial mycelium of the tra1- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. TRA1-deleted mutant. 4 biological replicates with dye-swaps. Each microarry slide had 2 arrays with 44k spots (22k spots printed twice). Thus, rep1 and rep2 are technical replicates of the same biological sample (derived from one raw data file).

2012-03-03 | E-GEOD-36224 | biostudies-arrayexpress

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Label-free quantitative proteomic analysis for identification of in-planta Magnaporthe oryzae proteins induced cell death

Project description:The rice blast disease caused by Magnaporthe oryzae (M.oryzae) is one of the most important disease in cultivated rice (Oryza sativa L.) results in extensive loss of productivity worldwide. Therefore, here we utilized the label-free quantitative proteomic approach to identify the novel M. oryzae effectors. A total of 114 were significantly modulated M. oryzae proteins derived from infected rice leaves. Among them, twenty-nine proteins were predicted as secreted proteins by SignalP 4.0. In addition, functional annotation proteins revealed that five proteins were mainly associated with hydrolase family. Consequently, five putative effector genes were cloned into a plant expression vector and fused with MYC tag in the C-terminal. Finally, we found that three in-planta M.oryzae hydrolase proteins were induced cell death after Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana (N.benthamiana). MGP1 (endo-1,4-beta-xylanase) and MGP4 (cellulose-binding protein) were localized in extracellular. Otherwise, MGP5 (cutinase) localized in intracellular space induced cell death. Thus, our results suggest that in-planta M. oryzae hydrolase proteins may play a key role in innate immunity via PAMP (Pathogen-Associated Molecular Pattern) in rice.

2022-04-04 | PXD026316 | Pride

Data-independent acquisition (DIA)-MS analysis for identification of Magnaporthe oryzae secreted protein induces cell death and triggers defense responses in rice

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2024-03-28 | PXD045543 | Pride

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