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Expression data from 11-day-old antisense BoEMF2.1 broccoli

ABSTRACT: EMF2 genes play an important function in inhibiting reproductive programs during vegetative stage of plant development. We knocked down the expression of endogenous EMF2 genes in broccoli by introduing a 35S::antisenseBoEMF2.1 contruct. The transgenic broccoli with knocking-down expression of endogenous EMF2s showed aberrant phenotypes during development. We used microarrays to study the global programme of gene expression and to identified genes misexpressed in broccoli with knocking-down expression of endogenous EMF2 genes 11-day-old seedlings of broccoli harboring the empty vector pCAMBIA1380 and that harboring 35S::antisenseBoEMF2.1 (asBoEMF2.1) were used for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Brassica oleracea var. italica

SUBMITTER: Mao-Sen Liu

PROVIDER: E-GEOD-36963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from 11-day-old antisense BoEMF2.1 broccoli

Project description:EMF2 genes play an important function in inhibiting reproductive programs during vegetative stage of plant development. We knocked down the expression of endogenous EMF2 genes in broccoli by introduing a 35S::antisenseBoEMF2.1 contruct. The transgenic broccoli with knocking-down expression of endogenous EMF2s showed aberrant phenotypes during development. We used microarrays to study the global programme of gene expression and to identified genes misexpressed in broccoli with knocking-down expression of endogenous EMF2 genes

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Project description:Arabidopsis emf2 mutants bypass vegetative development and flowering upon seed germination. We introduced a broccoli BoEMF2.1 gene into emf2 mutants and obtained rescued emf2 plants that harbored 35S::BoEMF2.1. We found that BoEMF2.1 can partially rescue the phenotype of emf2 to that of WT. We used microarrays to study the global program of gene expression and to identify genes misexpressed in the Arabidopsis emf2 mutant that had been rescued by 35S::BoEMF2.1. 7-day-old seedlings of WT, transWT, emf2 and rescued emf2 Arabidopsis were used for RNA extraction and hybridization on Affymetrix microarrays.

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Project description:Expression data from 7-day-old Arabidopsis emf2 mutant, rescued emf2 mutant harboring 35S::BoEMF2.1, WT Columbia ecotype and WT harboring 35S::BoEMF2.1 named transWT

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