Stress response study in Arabidopsis thaliana using DNA millichips

ABSTRACT: Our goal was to create a DNA chip that is as easy, convenient and inexpensive as an agarose gel. For a first generation solution, we describe a low cost, easy to use de novo synthesis oligonucleotide microarray technology that draws on the inherent flexibility of the maskless array synthesizer for in situ synthesis of thousands of photolithographically produced oligonucleotides covalently attached to a microscope slide. The method involves physically subdividing the slide into 1 x 1 mm 'millichips' that are hybridized to fluorescent RNA or DNA of biological origin, in a microfuge tube at an ordinary laboratory benchtop, rather than in dedicated hybridization chambers. Fluorescence intensity is then measured with a standard microxcope rather than sophisticated DNA chip scanners. For proo

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jamison Wolfer 

PROVIDER: E-GEOD-37118 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress