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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression in the dorsal telencephalon of E13.5 conditional Dicer KO mice

ABSTRACT: To study the effect of miRNA depletion in the embryonic forebrain, we isolated RNA from E13.5 dorsal telencephalon of mice homozygous for a floxed Dicer allele and hemizygous for the forebrain expressed Emx1Cre (Dicer KO mice). These samples were compared to littermates heterozygous for the floxed allele and hemizygous for Emx1Cre. 5 Dicerko mice and 4 controls, partly littermates, no technical replicates

ORGANISM(S): Mus musculus

SUBMITTER: Wolfgang Enard

PROVIDER: E-GEOD-37610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Convergent repression of Foxp2 3'UTR by miR-9 and miR-132 in embryonic mouse neocortex: implications for radial migration of neurons.

Clovis Yoanne M YM Enard Wolfgang W Marinaro Federica F Huttner Wieland B WB De Pietri Tonelli Davide D

Development (Cambridge, England) 20120808 18

MicroRNAs (miRNAs) are rapidly emerging as a new layer of regulation of mammalian brain development. However, most of the miRNA target genes remain unidentified. Here, we explore gene expression profiling upon miRNA depletion and in vivo target validation as a strategy to identify novel miRNA targets in embryonic mouse neocortex. By this means, we find that Foxp2, a transcription factor associated with speech and language development and evolution, is a novel miRNA target. In particular, we find ...[more]

PMID: 22874921

Similar Datasets

Expression in the dorsal telencephalon of E13.5 conditional Dicer KO mice

Project description:To study the effect of miRNA depletion in the embryonic forebrain, we isolated RNA from E13.5 dorsal telencephalon of mice homozygous for a floxed Dicer allele and hemizygous for the forebrain expressed Emx1Cre (Dicer KO mice). These samples were compared to littermates heterozygous for the floxed allele and hemizygous for Emx1Cre.

2012-08-30 | GSE37610 | GEO

Identification and validation of gene expression changes upon removal of Dicer in the murine limb mesenchyme

Project description:Wild type and Dicer null embryonic mouse limbs were analaysed using Affymetrix arrays to identify gene expression changes. Genes that were up-regulated in Dicer-null limbs were canidates for being miRNA targets. Potential miRNA target genes were validated using qRTPCR. Mice carrying a heterozygous Dicer floxed allele and the prxcre driver allele were crossed to homozygous Dicer Floxed mice. Embryos were harvested from pregent moms and genotyped to determine heterozygous, Wild type and mutant limb buds. These limb buds were used to prepare RNA for array analysis.

2011-01-31 | E-GEOD-13807 | biostudies-arrayexpress

Gene expression profile of Dicer knockout in mouse DP thymocytes

Project description:The RNase III enzyme dicer is essential for the processing of microRNAs (miRNAs) and small interfering RNAs (siRNAs) from double-stranded RNA precursors. miRNAs and siRNAs regulate chromatin structure, gene transcription, mRNA stability and translation in a wide range of organisms. To provide a model system to explore the role of dicer-generated RNAs in the differentiation of mammalian cells in vivo, we have generated a conditional dicer allele. Deletion of dicer at an early stage of T cell development compromised the survival of αβ lineage cells, while the numbers of γδ-expressing thymocytes were not affected. In developing thymocytes, dicer was not required for the maintenance of transcriptional silencing at pericentromeric satellite sequences (constitutive heterochromatin), the maintenance of cytosine DNA methylation and X chromosome inactivation in female cells (facultative heterochromatin) and the stable shutdown of a developmentally regulated gene (developmentally regulated gene silencing). Most remarkably, given that one-third of mammalian mRNAs are putative miRNA targets, dicer appears to be dispensable for CD4/8 lineage commitment, a process where epigenetic regulation of lineage choice has been well documented. Thus, although dicer appears critical for the development of the early embryo, it may have limited impact on the implementation of lineage-specific gene expression programs. LckCre was used to delete a floxed Dicer1 allele from developing thymocytes (Cobb BS, Nesterova TB, Thompson E, Hertweck A, O'Connor E, Godwin J, Wilson CB, Brockdorff N, Fisher AG, Smale ST and Merkenschlager M. T cell lineage choice and differentiation in the absence of the RNAse III enzyme dicer. J. Exp. Med. 2005 201: 1367-1373). Thymocytes were stained for CD4 and CD8, and double-positive (DP) thymocytes were sorted by flow cytometry. Three biological replicates of Dicer lox/lox control samples and LckCre Dicer lox/lox KO samples were analysed.

2015-01-28 | E-GEOD-57511 | biostudies-arrayexpress

Gene expression of colon from control or VilCre;Ihhflox/flox mice

Project description:Ihh expression is required for intestinal stem cell niche development. Loss of Ihh leads to disruption of intestinal stem cell niche, which leads to abnormal stem cell development and epithelial cell differentiation. 3 paired control (C) and mutant (M) mice here harvested. Their colon tissues were analyzed using Affymetrix Mouse Gene 1.0ST array Control and VilCre;Ihhflox/flox mice were harvested at p12 There are 3 pairs (total 6) samples: M1 and C1 are littermates M2 and C2 are littermates M3 and C3 are littermates

2010-06-16 | E-GEOD-18393 | biostudies-arrayexpress

Brain BRCA1 conditional knockout

Project description:BRCA1 nestin CRE conditional knockout cortrices of P7 animals were compared to wildtype littermates to characterize the mutant phenotype. Keywords: expression BRCA1 conditional knockouts using nestin CRE and a null allele with an inverted neo cassette at the 5' end of the exon 11 of BRCA1 on one floxed allele flanking exons 5-13. Cortices of 3 wildtype animals were compred to 3 BRCA cKO at postnatal day 7.

2011-09-07 | E-GEOD-6310 | biostudies-arrayexpress

Cyldko

Project description:Effect of Cyld inactivation in liver. The Cyld gene was conditionally inactivated specifically in the liver by crossing mice carrying floxed exon 9 allele of Cyld with Alfp-Cre transgenic mice. Liver RNAs from Cyld lox/lox-AlfpCre mice were compared with RNAs prepared from the livers of wild type littermates.

2012-02-01 | E-MEXP-3478 | biostudies-arrayexpress

Cohesin regulates gene expression through spatial clustering of enhancer elements

Project description:H3K27Ac ChIP-seq in wild type and cohesin-deficient thymocytes Rad21 was deleted in CD4+ CD8+ double positive (DP) thymocytes by crossing a Rad21 floxed allele with a Cd4-driven Cre transgene. DP positive thymocytes were FACS-sorted from control and Rad21-/- littermates, which were then used to perform chromatin immunoprecipitation for histone H3 acetylated on lysine 27 (H3K27Ac).

2015-02-12 | E-GEOD-61428 | biostudies-arrayexpress

Gene expression analysis of a podocyte specific PTIP deletion in mouse glomerular preparations at 1 month of age

Project description:Glomerular RNA comparison between wild-type and podocyte specific deletion of the PTIP gene in 1 month old kidneys. The PTIP gene was deleted using a floxed allele and a Podocin-Cre driver strain. These mice develop protein urea by 3 months of age. This study was designed to find gene expression differences prior to the onset of the phenotype. A ptip floxed allele was crossed into the Podocin-Cre expressing strain to generate ptip fl/fl homozygous mice or ptip fl/- transheterozygous mice with or without the Cre expressing transgene. Animals were genotyped and assigend into two groups: Mutants with PTIP deletion (PTIP-) with the genotype ptip fl/fl:Pod-Cre or ptip fl/-:Pod-Cre or control mice (PTIP+) with either ptip fl/fl or ptip fl/-. Note that the floxed allele produced normal levels of wild-type PTIP protein. Kidneys were excised at 1 month of age. Glomeruli enriched fractions were generated by sieving of the tissue homogenates. RNA was prepared from glomerular enriched fractions.

2009-08-19 | E-GEOD-17709 | biostudies-arrayexpress

Gene expression profile of HLRCC associated renal cell cancer

Project description:Gene expression profiles of normal kidney (3 technical replicates) and a renal tumor (3 technical replicates) from a hereditary leiomyomatosis and renal cell cancer (HLRCC) patient carrying a germline mutation in the fumarate hydratase (FH) gene. Tumor and normal tissue from one patient, both in 3 techincal replicates.

2010-12-31 | E-GEOD-20896 | biostudies-arrayexpress

Ileum single cells expression in mice lacking Pgc1α in the intestine in response to western diet feeding.

Project description:To generate iPGC1α-/- mice, PGC1αfl/fl mice were intercrossed with Vil1-Cre mice to obatin Vil1-CreTg/-PGC1αfl/- mice. These mice were then backcrossed with PGC1αfl/fl mice to restore homozygosity for the iPGC1α floxed allele. Male Vil1-CreTg/-PGC1αfl/fl mice were bred to PGC1αfl/fl to produce the mice used in the study. PGC1αfl/fl (iPGC1afl/fl) littermates were used as controls.

2023-11-02 | GSE227610 | GEO

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