Identification of tissue microRNAs predictive of sutinib activity in patients with metastatic renal cell carcinoma

ABSTRACT: Purpose: To identify tissue microRNAs predictive of sunitinib activity in patients with metastatic renal-cell carcinoma (MRCC) and to validate them in a cellular model. Selected microRNAs were studied in serum from MRCC patients and healthy individuals. Methods: We screened 673 microRNAs using TaqMan Low-density Arrays (TLDAs) in tumors from MRCC patients with extreme phenotypes of marked efficacy and resistance to sunitinib, selected from an identification cohort (n=41). Differentially expressed microRNAs were selected using bioinformatics-based target prediction analysis and quantified by qRT-PCR in tumors from patients presenting similar phenoytpes selected from an independent cohort (n=117). Results were validated in a cellular model of sunitinib resistance and studied in serum from h

ORGANISM(S): Homo sapiens

SUBMITTER: Alfonso Calvo 

PROVIDER: E-GEOD-37766 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress