Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Prolactin-inducible Stat5a-mediated and Stat5b-mediated gene profiles

ABSTRACT: Stat5a and Stat5b proteins are highly homologous with greater than 90% amino acid identity and share binding to the palindromic Stat5 consensus sequence, TTCNNNGAA, but individual roles of each transcription factor in breast cancer have not been thoroughly evaluated. To determine the degree of similarity between transcripts modulated by Stat5a and Stat5b proteins in human breast cancer, we utilized genome-wide transcript profiling to identify genes regulated specifically by Stat5a or Stat5b in response to prolactin. Stat5a or Stat5b was transiently overexpressed using adenoviral gene delivery in MCF7 breast cancer cells followed 16 hr serum starvation and a brief 4 hr exposure to 10nM human prolactin to identify immediate-early transcripts modulated by each transcription factor. Basal activation of Stat5a or Stat5b was not present in cells not stimulated with prolactin. mRNA from each condition was harvested and validated using the Agilent bioanalyzer. cDNA was generated and genome-wide transcript profiling was performed in triplicate using the Affymetrix HuGene 1.0 ST array.

ORGANISM(S): Homo sapiens

SUBMITTER: Adam Ertel 

PROVIDER: E-GEOD-37781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

<h4>Introduction</h4>Signal transducer and activator of transcripton-5a (Stat5a) and its close homologue, Stat5b, mediate key physiological effects of prolactin and growth hormone in mammary glands. In breast cancer, loss of nuclear localized and tyrosine phosphorylated Stat5a/b is associated with poor prognosis and increased risk of antiestrogen therapy failure. Here we quantify for the first time levels of Stat5a and Stat5b over breast cancer progression, and explore their potential associatio  ...[more]

Similar Datasets

Unknown Prolactin-inducible Stat5a-mediated and Stat5b-mediated gene profiles
2012-08-29 | GSE37781 | GEO
Genomics Impact of STAT5 dosage and binding location on genetic and biological programs activated in mouse mammary epithelium during pregnancy.
2012-09-18 | E-GEOD-40930 | biostudies-arrayexpress
Unknown Impact of STAT5 dosage and binding location on genetic and biological programs activated in mouse mammary epithelium during pregnancy.
2012-09-18 | GSE40930 | GEO
Unknown Prolactin Drives a Dynamic STAT5A/HDAC6/HMGN2 Cis-Regulatory Landscape Exploitable in ER+ Breast Cancer
2021-01-26 | GSE165452 | GEO
Genomics,Multiomics Homo sapiens
| PRJNA163267 | ENA
Unknown RNA-seq data from wildtype, STAT5A-knockout and STAT5B-knockout BCR/ABLp185+ transformed mouse bone marrow cell lines
2019-10-01 | GSE121246 | GEO
Genomics,Multiomics E-GEOD-37781
2015-08-18 | E-GEOD-37781 | ExpressionAtlas
Transcriptomics RB pathway deregulation promotes invasion and disease progression in a mouse model of MYC-overexpressing mammary tumorigenesis
2013-08-01 | E-GEOD-40545 | biostudies-arrayexpress
Transcriptomics Expression data MCF10A/ErbB2 RB-proficient and RB-deficient cells cultured in 3-D cell culture
2013-03-01 | E-GEOD-36425 | biostudies-arrayexpress
Unknown RNA sequencing data from MCF7 luminal breast cancer cells expressing STAT5a phospho-point mutants
2021-07-07 | GSE165678 | GEO