Innate immune pathways in afferent lymph following vaccination with poly(I:C) -containing liposomes

ABSTRACT: Many modern vaccines use defined adjuvants to stimulate the innate immune system and shape the adaptive immune response. The exact nature of these innate signals and whether immune differentiation can originate within the periphery is not known. In the present study we used an ovine lymphatic cannulation model to characterise the cellular and transcriptomic profile of the afferent lymph following injection of a liposomal vaccine formulation incorporating diphtheria toxoid and the innate stimulator poly (I:C) over a 78 h period. The response to this vaccine featured an early activation of broad proinflammatory pathways (e.g. TLR signalling and inflammasome pathways) and the transient recruitment of granulocytes into the lymph. At 24hrs a more monocytic cellular profile arose coinciding with

ORGANISM(S): Ovis aries

SUBMITTER: Melissa Burke 

PROVIDER: E-GEOD-38533 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress