Targeted RT-PCR assays spanning unannotated splice junctions sequenced by Roche 454.

ABSTRACT: The ENCODE projects seeks to identify and characterize functional elements in the human genome. Throughout the scale-up phase of ENCODE, the transcriptome group has generate Long RNA-Seq, Small RNA-Seq, Cap-Analysis of Gene Expression (CAGE), and RNA-PET short read data on the Illumina platform for ~ 40 different human primary and transformed cell lines in replicate. From these data several high-resolution and discrete features/elements have been mined out (5’ caps, splice junctions, polyadenylation sites, small RNAs, etc…). However, because these data are obtained from short-read data, we have only limited “connectivity” information. For example, from the long RNA-Seq data, which was sequenced in mate-pair fashion with average insert sizes ~ 200 bp, we know that the sequence from mat

ORGANISM(S): Homo sapiens

SUBMITTER: julien lagarde 

PROVIDER: E-GEOD-38886 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress