LoRTIS: A long read method for analysis of large transposon mutant libraries

ABSTRACT: Transposon insertion site sequencing (TIS) is a powerful method for associating genotype to phenotype. However, all TIS methods described to date use short nucleotide sequence reads which cannot uniquely determine the locations of transposon insertions within repeating genomic sequences where the repeat units are longer than the sequence read length. To overcome this limitation, we have developed a TIS method using Oxford Nanopore sequencing technology that generates and uses long nucleotide sequence reads; we have called this method LoRTIS (Long Read Transposon Insertion-site Sequencing). This experiment data contains sequence files generated using Nanopore and Illumina platforms. Biotin1308.fastq.gz and Biotin2508.fastq.gz are fastq files generated from nanopore technology. Rep1-Tn.fastq

INSTRUMENT(S): MinION, NextSeq 500

ORGANISM(S): Escherichia coli BW25113

SUBMITTER: Muhammad Yasir 

PROVIDER: E-MTAB-11351 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress