Leaf transcriptome comparison between wt and tut1 (salk_087647) -Transcriptome comparison between WT and a mutant impaired in RNA uridylation.
Ontology highlight
ABSTRACT: rs11-10_tut1 - leaf transcriptome comparison between wt and tut1 (salk_087647) - Identification of mRNA targets of the terminal uridylyltransferase TUT1 (AT2G45620) in Arabidopsis thaliana. - Identification of the RNA targets of the terminal uridylyl transferase TUT1 (AT2G45620). Mature rosettte leaf transcriptomes were compared between WT and a tut1 knock-out mutant (SALK_087647). 3 dye-swap - gene knock out
Project description:au10-06_salmonella_eduardob - salmonella_14028s - Transcriptomic analysis between Salmonella wt and differents mutants in type3 secretion system - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h, at 9 am samonella was added to a final concentration of OD600=0.2. Keywords: normal vs disease comparison 2 dye-swap - CATMA arrays
Project description:au10-01_overexpressionoxi1 - au10-01_overexpressionoxi1 - Analysis of the overexpression of the Oxi1 - Comparision between the overexpression of oxi1 and wt. 2 dye-swap - normal vs transgenic comparison
Project description:au10-06_salmonella_eduardob - inva_vs_14028s Transcriptomic analysis between Salmonella wt and different mutants in type3 secretion system. - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h. at 9 am samonella was added to a final concentration of OD600=0.2 2 dye-swap - normal vs disease comparison
Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction 6 dye-swap - gene knock out
Project description:ra09-02_tctp - over expression - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of 35S::AtTCTP versus wild type Keywords: gene knock in (transgenic) 2 dye-swap - CATMA arrays
Project description:We investigated which signaling pathways downstream of Gas6/Axl promote the invasive phenotype of liver cancer cells. Thus, we performed phospho-proteomic analysis of MR hepatocytes and those expressing Axl (MR-Axl) in the presence and absence of Gas6. Protein-clustering based on the pattern of phosphorylation across the conditions and samples revealed four clusters: phospho-sites that are upregulated dependent on Axl expression (cluster 1) or Gas6 expression (cluster 2) and phospho-sites that are downregulated dependent on Axl expression (cluster 3) or Gas6 expression (cluster 4). Functional enrichment analysis showed enrichment of protein sets associated with cellular polarity and motility depending on changes in Axl expression and Gas6 activation. Furthermore, we analyzed phospho-proteomic data to estimate the activity of kinases based on kinase-substrate interactions using PhosR. Kinase perturbation analysis indicated mammalian target of rapamycin (mTOR) as the one with the highest upregulated activity in Gas6-stimulated MR-Axl cells compared to Gas6-stimulated MR cells. Accordingly, we analyzed substrates of mTOR and found that phosphorylation of Akt1 (Ser473) was upregulated in cluster 1 and highly increased in Gas6-stimulated MR-Axl, suggesting that Akt is regulated by Axl.
Project description:ra09-02_tctp - rnai - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of 35S::RNAi-AtTCTP versus wild type Keywords: normal vs rnai mutant comparison 2 dye-swap - CATMA arrays
Project description:ra09-02_tctp - knockout - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of tctp-2 knock out versus wild type Keywords: gene knock out 2 dye-swap - CATMA arrays
Project description:Transcript profiling to identify genes where the mRNA expression levels are differentially regulated in white adipose tissue of transgenic mice overexpressing the FOXC2 gene compared with adipose tissue of wild-type mice.