Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression Response to Interferon Treatment at 12h and 24 in A549 cells

ABSTRACT: The goal of this experiment was to use global gene expression profiling to understand human lung epithelial cellular responses to interferon treatment. A549 cells were treated individually with IFN-α (10 ng ml−1; Sigma I4276), IFN-β (500 U ml−1; Sigma I4151) and IFN-γ (10 ng ml−1; Sigma I32265) or mock-treated. At 12 and 24 h post-treatment, cells were harvested (n = 3 wells per condition) and total RNA was extracted and analysed by microarray. Individual well replicates were pooled for each array by combining equal masses of total RNA (n=1 array per time point for each treatment and control).

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Green

PROVIDER: E-GEOD-39199 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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