Complexity of Murine Cardiomyocyte miRNA Biogenesis, Sequence Variant Expression and Function

ABSTRACT: microRNAs (miRNAs) are critical to heart development and disease. Emerging research indicates that regulated precursor processing can give rise to an unexpected diversity of miRNA variants. We subjected small RNA from murine HL-1 cardiomyocyte cells to next generation sequencing to investigate the relevance of such diversity to cardiac biology. ~40 million tags were mapped to known miRNA hairpin sequences as deposited in miRBase version 16, calling 403 generic miRNAs as appreciably expressed. Hairpin arm bias broadly agreed with miRBase annotation, although 44 miR* were unexpectedly abundant (>20% of tags); conversely, 33 -5p/-3p annotated hairpins were asymmetrically expressed. Overall, variability was infrequent at the 5M-bM-^@M-2 start but common at the 3M-bM-^@M-2 end of miRNAs (5.2% a

ORGANISM(S): Mus musculus

SUBMITTER: Hardip Patel 

PROVIDER: E-GEOD-39531 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress