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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Isw2 and Ume6 ChIP-chip in transcription factor deletion (M-NM-^Tume6, M-NM-^Tnrg1, M-NM-^Tcin5, and M-NM-^Tsok2) and DNA-looping deficient (sua7-1) cells

ABSTRACT: The sequence-specific transcription factors Ume6, Nrg1, Cin5, and Sok2 and the general transcription factor TFIIB mediate the genome-wide targeting of the ATP-dependent chromatin remodeling enzyme Isw2. At least two biological replicates comparing Isw2-ChIP (Isw2-K215R ChIP DNA competitively hybridized against WT Isw2 ChIP DNA) in WT and M-NM-^Tume6, M-NM-^Tnrg1, M-NM-^Tcin5, M-NM-^Tsok2, and sua7-1 strains or Ume6-ChIP (ChIP DNA competitively hybridized again Input DNA) in WT and sua7-1 strains

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Toshio Tsukiyama

PROVIDER: E-GEOD-39542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA looping facilitates targeting of a chromatin remodeling enzyme.

Yadon Adam N AN Singh Badri Nath BN Hampsey Michael M Tsukiyama Toshio T

Molecular cell 20130307 1

ATP-dependent chromatin remodeling enzymes are highly abundant and play pivotal roles regulating DNA-dependent processes. The mechanisms by which they are targeted to specific loci have not been well understood on a genome-wide scale. Here, we present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment. Unexpectedly, Isw2 is recruited in a TF-dependent fashion to a ...[more]

PMID: 23478442

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