Time course assay in Mouse Pre-Somitic Mesoderm

ABSTRACT: Stem cell-derived tissues have wide potential for modelling developmental and pathological processes as well as cell-based therapy. However, it has proven difficult to generate several key cell types in vitro, including skeletal muscle. In vertebrates, skeletal muscles derive during embryogenesis from the presomitic mesoderm (PSM). Treatment of mouse ES cells with a combination of the secreted Wnt activator R-Spondin3 and the BMP inhibitor Noggin generated cells expressing the early PSM marker Mesogenin1 (Msgn1) with high efficiency. To confirm their identity, we mapped gene expression profiles at successive stages of PSM differentiation in vivo and showed that the differentiated ES cells closely corresponded to the posterior PSM domain that expresses Msgn1 and then with time in culture m

ORGANISM(S): Mus musculus

SUBMITTER: philippe moncuquet 

PROVIDER: E-GEOD-39613 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress