Expression profiling of Arabidopsis wild type Col-0 and pmr5 mutant
Ontology highlight
ABSTRACT: The rosettes from genotype Col-0 and pmr5 mutant were collected for RNA expression profiling when the plants were 3 weeks old. The rosettes were collected when the plants were 3 weeks old for RNA extraction and hybridization on Affymetrix microarrays.
Project description:The rosettes from genotype Col-0, pmr5, pmr6 and pmr5 pmr6 double mutant were collected for RNA expression profiling when the plants were 3 weeks old. Experiment Overall Design: The rosettes were collected when the plants were 3 weeks old for RNA extraction and hybridization on Affymetrix microarrays.
Project description:How do the transcript levels of leaf-expressed genes change in a normal day-night cycle? The interest is in genes that are regulated by the circadian clock and the diurnal component (i.e. light, metabolite changes). Plants were grown on soil in a 12/12 h light/dark rythm at 20°C day and night. 5 weeks after germination the rosettes of the non-flowering plants were harvested, 15 plants per sample. Plants were harvested at 6 timepoints every 4 hours beginning with the end of the night (still in darkness). Keywords: repeat
Project description:The rosettes from genotype Col-0, pmr5, pmr6 and pmr5 pmr6 double mutant were collected for RNA expression profiling when the plants were 3 weeks old. Keywords: cell wall mutant
Project description:Identification of differential gene expression during RPS2-mediated effector-triggered immunity in Arabidopsis Four-week-old leaves of Arabidopsis Col-0 wild type and rps2 mutant plant were infiltrated with Pseudomonas syringae pv. Maculicola carring effector AvrRpt2 at OD 0.01. Samples were collected at 0, 6 and 10 hours after infiltration for WT plants and at 0 and 10 hours after infiltration for rps2 plants. Three biological replicates were performed per genotype per time point.
Project description:How do transcript levels of leaf-expressed genes change in a normal day-night cycle of the phosphoglucomutase (pgm) mutant? The interest is in genes that are regulated by the circadian clock and the diurnal component (i.e. light, metabolite changes). Plants were grown on soil in a 12/12 h light/dark rhythm at 20°C day and night. 5 weeks after germination the rosettes of the non-flowering plants were harvested, 15 plants per sample. Plants were harvested in series at 6 times every 4 hours, beginning with the end of the night (still in darkness). Keywords: repeat
Project description:The role of abscisic acid (ABA) signalling in the ascorbic acid (AA)-dependent control of plant growth and defence was determined using the vtc1 and vtc2 mutants, which have impaired ascorbic acid synthesis, and in the abi4 mutant that is impaired in ABA-signalling. ABA levels were increase in the mutants relative to the wild type (Col0). Like vtc1 the vtc2 mutants have a slow growth relative to Col0. However, the wild type phenotype is restored in the abi4vtc2 double mutant. Similarly, the sugar sensing phenotype of in the abi4 is reversed in the abi4vtc2 double mutant. The vtc1 and vtc2 leaf transcriptomes show up to 70 % homology with abi4. Of the transcripts that are altered in the mutants a relative to Col0, only a small number are reversed in the abi4vtc2 double mutants relative to either abi4 or vtc2. We conclude that AA controls growth via an ABA and abi4-dependent signalling pathway. The vtc and abi4 mutants have enhanced glutathione levels and common redox signalling pathways leading to similar gene expression patterns. Rosettes of 42 days old plants were harvested and used to exctract RNA
Project description:Identification of AvrRpt2-mediated differential gene expression in Arabidopsis Four-week-old leaves of Arabidopsis Col-0 plant were infiltrated with Pseudomonas syringae pv. Maculicola carring effector AvrRpt2 at OD0.01. Samples were collected at 0 or 6 hours after infiltration. Each time point contains three biological replicates.
Project description:The 4 weeks old plant of genotype overexpression mil4 mutants were sprayed with BTH. The rosette tissue were collected for RNA expression profiling. Experiment Overall Design: The rosettes were collected when the plants were 4 weeks old for RNA extraction and hybridization on Affymetrix microarrays.
Project description:The edr1 mutant of Arabidopsis thaliana displays enhanced resistance to the powdery mildew Golovinomyces cichoracearum, resulting in cell death and an absence of visible disease symptoms. To better characterize and understand the defense response of edr1, a time course of early signaling responses was performed after inoculation with powdery mildew and compared to the responses of wild-type Col-0. These time points represent early stages in the infection process, before any signs of susceptibility or resistance are visible. Four-week-old Col-0 and edr1 plants were inoculated with powdery mildew and whole rosettes were collected at 0, 18, 36, and 96 hours post inoculation. Each sample is a pool of four rosettes processed together.