TNFalpha and IL1beta stimulate differential gene expression in endometrial stromal cells

ABSTRACT: Cytokines are implicated in the development of inflammatory diseases such as endometriosis. This project was designed to test the hypothesis that specific cytokines that are secreted by macrophages, such as tumor necrosis factor ? (TNF?) and interleukin 1 beta (IL1?), cause gene expression changes in endometrial stromal cells. Telomerase-immortalized human endometrial stromal cells (T-HESC) were treated with TNF? (5ng/ml) ± IL1? (1ng/ml). DNA microarray and real time RT-PCR were used to study the gene expression changes in T-HESC cells. Two hundred and nineteen genes featuring in various gene ontologies were found to be differentially expressed in T-HESC cells treated with TNF? ± ILI?. The gene ontologies included functions expected to be associated with the development of endometriosis su

ORGANISM(S): Homo sapiens

SUBMITTER: Kathleen Eyster 

PROVIDER: E-GEOD-40007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress