Pol II docking and pausing at growth and stress genes in C. elegans

ABSTRACT: Fluctuations in nutrient availability profoundly impact gene expression. Previous work revealed post-recruitment regulation of RNA Polymerase II (Pol II) during starvation and recovery in Caenorhabitis elegans, suggesting promoter-proximal pausing promotes rapid response to feeding. To test this hypothesis, we measured Pol II elongation genome-wide by two complementary approaches and analyzed elongation in conjunction with Pol II binding and expression. We confirmed bona fide pausing during starvation and also discovered Pol II docking. Pausing occurs at active stress-response genes that become down-regulated in response to feeding. In contrast M-bM-^@M-^\dockedM-bM-^@M-^] Pol II accumulates without initiating upstream of inactive growth genes that become rapidly up-regulated upon feedin

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Colin Maxwell 

PROVIDER: E-GEOD-40161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress