Multiple roles for LEC in initiation and elongation phases of snRNA gene transcription

ABSTRACT: The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to unique aspects of their promoter structure, selectivity for either RNA Polymerase (Pol) II or III and a unique mechanism of termination that is tightly linked with the promoter. Recently, we identified the Little Elongation Complex (LEC) in Drosophila that is required for the expression of Pol II-transcribed snRNA genes. Here, we identify the molecular mechanism by which LEC specifically regulates Pol II-dependent snRNA gene transcription. We present genetic and molecular evidence from both Drosophila and mammals that LEC regulates both initiation and elongation stages of transcription of Pol II-transcribed snRNA genes. In human HCT116 cells we performed: ChIP-se

ORGANISM(S): Homo sapiens

SUBMITTER: Alexander Garruss 

PROVIDER: E-GEOD-47938 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress