JeB001: Elucidation of the distal convoluted tubule transcriptome identified new candidate players in magnesium balance

ABSTRACT: The distal convoluted tubule (DCT) plays a key role in the fine-tuning of renal Mg2+ reabsorption. In recent years, hereditary magnesium (Mg2+) transport disorders have helped to identify important players in DCT Mg2+ homeostasis. Here, we report the Mg2+-sensitive transcriptional expression of the DCT transcriptome using gene expression microarray analysis. Mice expressing eGFP under a DCT-specific promoter were subjected to Mg2+-deficient or Mg2+-enriched diets. Subsequently, the Complex Object Parametric Analyzer and Sorter (COPAS) allowed isolation of eGFP-positive DCT cells. RNA extracts were subjected to pair-wise microarray analysis, high vs. low Mg2+. Of all genes in the genome, 46 were shown to be differentially expressed with a minimal fold-change of 2. Several known magnesiotrop

ORGANISM(S): Mus musculus

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-GEOD-40208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress