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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of gene content among Group II Clostridium botulinum strains

ABSTRACT: The purpose of this study was to compare the gene content of several group II C. botulinum strains to determine the utility of DNA microarrays for subtyping this organism. Group II type B, E, and F C. botulinum strains were compared in a study to examine the gene content of a unique type E strain (CDC66177). One replicate of genomic DNA isolated from 16 type E strains, 4 type B strains, and 2 type F strains were examined.

ORGANISM(S): Clostridium botulinum

SUBMITTER: Brian Raphael

PROVIDER: E-GEOD-40271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype.

Raphael Brian H BH Lautenschlager Matthew M Kalb Suzanne R SR de Jong Laura I T LI Frace Michael M Lúquez Carolina C Barr John R JR Fernández Rafael A RA Maslanka Susan E SE

BMC microbiology 20121031

<h4>Background</h4>Clostridium botulinum strains that produce botulinum neurotoxin type E (BoNT/E) are most commonly isolated from botulism cases, marine environments, and animals in regions of high latitude in the Northern hemisphere. A strain of C. botulinum type E (CDC66177) was isolated from soil in Chubut, Argentina. Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA ope ...[more]

PMID: 23113872

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Analysis of gene content among Group II Clostridium botulinum strains

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Clostridium botulinum

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Use of CGH microarrays to identify strain variable genomic regions in C. botulinum ATCC 3502

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