Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from H226 squamous cell carcinoma cells

ABSTRACT: To define the contribution of p53 versus DNp63a to gene expression control, we performed a series of microarray assays for cells where p53 was activated using Nutlin-3 or DNp63a was knocked down using a stably transduced tetracycline inducible shRNA. We determined that p53 transcriptionally activates and DNp63a transcriptionally represses a non-overlapping subset of genes. Total RNA from H226 cells harvested using Tri reagent (Sigma-Aldrich) was used for gene expression analysis on Affymetrix HuGene 1.0 ST arrays following the manufacturer’s instructions. Differential gene expression was determined with Partek software using one-way ANOVA.

ORGANISM(S): Homo sapiens

SUBMITTER: Corrie Gallant-Behm 

PROVIDER: E-GEOD-40462 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ΔNp63α represses anti-proliferative genes via H2A.Z deposition.

Gallant-Behm Corrie L CL   Ramsey Matthew R MR   Bensard Claire L CL   Nojek Ignacio I   Tran Jack J   Liu Minghua M   Ellisen Leif W LW   Espinosa Joaquín M JM  

Genes & development 20120926 20

ΔNp63α is a member of the p53 family of transcription factors that functions as an oncogene in squamous cell carcinomas (SCCs). Because ΔNp63α and p53 bind virtually identical DNA sequence motifs, it has been proposed that ΔNp63α functions as a dominant-negative inhibitor of p53 to promote proliferation and block apoptosis. However, most SCCs concurrently overexpress ΔNp63α and inactivate p53, suggesting the autonomous action of these oncogenic events. Here we report the discovery of a novel mec  ...[more]

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