Identification of transcription factor binding sites from ChIP-seq data at high-resolution

ABSTRACT: Genome-wide mapping of proteinM-bM-^@M-^SDNA interactions is essential for a full understanding of transcriptional regulation. A precise map of binding sites for transcription factors, core transcriptional machinery is vital for deciphering the gene regulatory networks that underlie various biological processes. Chromatin immunoprecipitation followed by sequencing (ChIPM-bM-^@M-^Sseq) is a technique for genome-wide profiling of DNA-binding proteins. However, our conventional ChIPM-bM-^@M-^Sseq occasionally gives wider peaks which might be due to overlapping binding sites of two or more transcription factors. Therefore, to improve the resolution of our conventional ChIPM-bM-^@M-^Sseq which have DNA-protein footprint of ~100 bp, we decreased the size of DNA-protein footprint to ~ 50 bp by DN

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Samuel Meier 

PROVIDER: E-GEOD-40664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress