Transcriptional and Epigenetic Dynamics During Specification of Human Embryonic Stem Cells

ABSTRACT: Differentiation of human embryonic stem cells (hESCs) provides a unique opportunity to study the epigenetic mechanisms that facilitate cellular transitions in a human context. To that end, we performed comprehensive transcriptional and epigenetic profiling of early populations derived through directed differentiation of hESCs representing each of the three embryonic germ layers. Integration of whole genome bisulfite sequencing, chromatin immunoprecipitation-sequencing and RNA-Sequencing reveals unique events associated with specification towards each lineage. While we observe expected dynamics such as loss of DNA methylation and gain of H3K4me1 at distal putative regulatory elements, we frequently found a germ layer specific switch to H3K27me3 at sites of high DNA methylation in the undiff

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Ziller 

PROVIDER: E-GEOD-46130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress