ERK signaling regulates opposing functions of JUN family transcription factors in prostate cancer cell migration

ABSTRACT: Knockdowns of c-JUN and JUND had opposite effects on PC3 prostate cell migration. We predicted that c-JUN and JUND control the same set of cell migration genes, but in opposite directions. To test this hypothesis, mRNA with expression changes in c-JUN and JUND knockdown PC3 cell lines were compared to mRNA levels in control (luciferase knockdown) PC3 cells by RNA-seq. mRNA profiles of luciferase knockdown (WT), c-Jun knockdown, and Jun-D knockdown in PC3 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Hollenhorst 

PROVIDER: E-GEOD-53470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress