Quantitative, genome-wide analysis of eukaryotic replication initiation and termination

ABSTRACT: Via the deep-sequencing of Okazaki fragments from Saccharomyces cerevisiae, we report the first comprehensive documentation of genome-wide replication directionality in any eukaryote; this permits the systematic analysis of both replication initiation and termination. We conduct a genome-wide analysis of origin competence and efficiency, and conclude that the majority of origins are competent to fire in each cell cycle and generally do so with high efficiency. Additionally, the spatial resolution of our data allow us to determine that leading-strand initiation generally occurs within the nucleosome-free region at origins. Using a strain in which late origins can be induced to fire early, we show that replication termination is a largely passive phenomenon that does not rely on cis-acting s

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Iestyn Whitehouse 

PROVIDER: E-GEOD-40696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress