Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Joshua Levin
PROVIDER: E-GEOD-40705 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Nature methods 20130519 7
RNA-seq is an effective method for studying the transcriptome, but it can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations or cadavers. Recent studies have proposed several methods for RNA-seq of low-quality and/or low-quantity samples, but the relative merits of these methods have not been systematically analyzed. Here we compare five such methods using metrics relevant to transcriptome annotation, transcript discovery and gene expression. Using ...[more]