Genome-wide mapping of nucleosome positioning and DNA methylation within Individual DNA molecules

ABSTRACT: DNA methylation and nucleosome positioning work together to generate chromatin structures that regulate gene expression. Nucleosomes are typically mapped using nuclease digestion requiring significant amounts of material and varying enzyme concentrations. We have developed a method (NOMe-seq) that uses a GpC methyltransferase (M.CviPI) and next generation sequencing to generate a high resolution footprint of nucleosome positioning genome-wide using less than 1 million cells while retaining endogenous DNA methylation information from the same DNA strand. Using a novel bioinformatics pipeline we show a striking anti-correlation between nucleosome occupancy and DNA methylation at CTCF regions, that is not present at promoters. We further show that the extent of nucleosome depletion at promote

ORGANISM(S): Homo sapiens

SUBMITTER: Yaping Liu 

PROVIDER: E-GEOD-40770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress