Genome-wide comparison of DNA hydroxymethylation in mouse embryonic stem cells and neural progenitor cells by relative quantitative hMeDIP-seq

ABSTRACT: DNA hydroxymethylation (5hmC) represents a new layer of epigenetic regulation in addition to DNA methylation (5mC). The genome-wide patterns of 5hmC distribution in many tissues and cells have recently been revealed by hydroxymethylated DNA immunoprecipitation (hMeDIP) followed by high throughput sequencing or tiling arrays. However, the DNA hydroxymethylome data generated by the conventional hMeDIP-seq method can not be used for direct quantitative comparison across different samples. In this study, we report a new quantitative hMeDIP-seq method, which uses barcode technology to label different DNA samples and performs hMeDIP-seq for multiple samples in one reaction system. Using this new method, we profiled and compared the DNA hydroxymethylomes of mouse ES cells (ESCs) and mouse ESCs-de

ORGANISM(S): Mus musculus

SUBMITTER: Wenqi Xu 

PROVIDER: E-GEOD-40810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress