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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome profiles of brain glioma stem cells and normal neural stem cells

ABSTRACT: A human cDNA microarray comprising 29,187 human genome probes was used to evaluate the transcriptional changes between brian glioma stem cells (GSC) and normal neural stem cells(NSC). There were 6 samples that were analyzed, 3 samples were from glioma stem cells and the other 3 samples form normal neural stem cells were controls. cell type: brain glioma stem cells:GSC_H004, GSC_H005, GSC_H006 ; cell line: normal neural stem cells: NSC_H001, NSC_H002, NSC_H003 biological replicate: NSC_H001, NSC_H002, NSC_H003; biological replicate: GSC_H004, GSC_H005, GSC_H006

ORGANISM(S): Homo sapiens

SUBMITTER: Shuang Liu

PROVIDER: E-GEOD-41031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulatory roles of miRNA in the human neural stem cell transformation to glioma stem cells.

Liu Shuang S Yin Feng F Zhang Jianning J Wicha Max S MS Chang Alfred E AE Fan Wenhong W Chen Ling L Fan Ming M Li Qiao Q

Journal of cellular biochemistry 20140801 8

To investigate the expressional alternation of microRNAs (miRNA) during the malignant transformation and development of human glioma, we measured miRNA expression profile as well as mRNA expression profile in normal human neural stem cells (hNSCs) and human glioma stem cells (hGSCs). We found 116 miRNA up-regulated and 62 miRNA down-regulated in GSCs. On the other hand, we identified 1,372 mRNA down-regulated, and 1,501 mRNA up-regulated in GSCs compared to those in NSCs. We then analyzed the pa ...[more]

PMID: 24519663

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Transcriptome profile of peripheral blood mononuclear cells in children with acute otitis media caused by Streptococcus pneumoniae

Project description:Streptococcus pneumoniae (Spn) is the predominant causative organism of acute otitis media (AOM) in children. A human cDNA microarray comprising 30,968 human genome probes was used to evaluate the transcriptional changes that occur in peripheral blood mononuclear cells (PBMC) at the onset of clinical AOM caused by Spn infection in children after comparison of microarray results with the pre-infection healthy stage of the same children. Four to ten milliliters of heparinized peripheral venous blood was collected from children at 6 to 30 months of age when they were in acute otitis media (AOM) stage and pre-infection healthy stage. The diagnosis of AOM was based on symptoms and signs as well as S. pneumoniae culture positive in the middle ear fluid. Patients with polymicrobial infections, history of immunodeficiency, history of chronic or recurrent AOM, chronic disease, or receiving steroids or other immunomodulatory agents were excluded. Peripheral blood mononuclear cells (PBMCs) were isolated by the Ficoll gradient and total RNA was extracted from PBMCs using the QIAamp RNA blood Mini Kit (Qiagen, Maryland, USA) according to manufacturer’s instructions. Double-stranded cDNA generated from total RNA was labeled with Cyanine-5 and subsequently hybridized to Human OneArray glass slides according to the manufacturer's standard protocols (PhalanxBio Inc, CA, USA). Microarrays were scanned at 5 μm resolution using an Agilent scanner. Raw intensity signals for each microarray were captured using a Molecular Dynamics Axon 4100A scanner, measured using GenePixPro™ Software. The data from all microarrays in each experimental set was then analyzed using Omicsoft Array Studio software; control and missing features were removed, and the remaining signals were quantile normalized.

2010-07-26 | E-GEOD-23140 | biostudies-arrayexpress

Transcriptome profile of peripheral blood mononuclear cells in children with acute otitis media caused by Nontypeable Haemophilus influenzae

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2012-03-15 | E-GEOD-27990 | biostudies-arrayexpress

Brain glioma stem cells and normal neural stem cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-07-30 | E-GEOD-41033 | biostudies-arrayexpress

miRNA profiles of brain glioma stem cells and normal neural stem cells

Project description:A human microarray comprising 1658 human genome probes was used to evaluate the specific expression of miRNA between brian glioma stem cells (GSC) and normal neural stem cells(NSC). 2 total RNA samples are analyzed, brian glioma stem cells (GSC) and normal neural stem cells(NSC). cell type: brain glioma stem cells:GSC_1, GSC_2, GSC_3 ; cell line: normal neural stem cells: NSC_1, NSC_2, NSC_3 biological replicate: NSC_1, NSC_2, NSC_3; biological replicate: GSC_1, GSC_2, GSC_3

2014-07-30 | E-GEOD-41032 | biostudies-arrayexpress

Development of microRNA (miRNA) expression signatures for human glioma-initiating cells (GICs)

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2015-04-07 | E-GEOD-61079 | biostudies-arrayexpress

An Artificial miRNA Against HSPE Suppresses Melanoma Invasion Properties Correlating with a Down-regulation of Chemokines and MAPK Phosphorylation

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2012-10-27 | E-MEXP-3443 | biostudies-arrayexpress

Gene expression profile of A549 Circulating Tumor Cells from 4D Model.

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2015-03-12 | E-GEOD-50991 | biostudies-arrayexpress

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2009-11-30 | E-GEOD-17062 | biostudies-arrayexpress

Ets factors regulate neural stem cell depletion and gliogenesis in Ras pathway-driven glioma

Project description:As the list of putative driver mutations in glioma grows, we are just beginning toAs the list of putative driver mutations in glioma grows, we are just beginning to elucidate the effects of dysregulated developmental signaling pathways on the transformation of neural cells. We have employed a postnatal, mosaic, autochthonous, glioma model that captures the first hours and days of gliomagenesis in more resolution than conventional genetically engineered mouse models of cancer. We provide evidence that disruption of the Nf1-Ras pathway in the ventricular zone at multiple signaling nodes uniformly results in rapid neural stem cell depletion, progenitor hyperproliferation, and gliogenic lineage restriction. Abolishing Ets subfamily activity, which is upregulated downstream of Ras, rescues these phenotypes and blocks glioma initiation. Thus, the Nf1-Ras-Ets axis might be one of the select molecular pathways that are perturbed for initiation and maintenance in glioma. Mouse Gene 1.0 ST Microarrays from Affimetryx were used for microarray analysis. For each experiment, total RNA was fluorescently labeled and hybridized. 3 cell lines derived from in vivo electroporated neural stem and progenitors were compared.

2015-05-28 | E-GEOD-69254 | biostudies-arrayexpress

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