Comparative Genomic Hybridization of Leishmania major SbIII resistant mutants and L. major WT

ABSTRACT: SbIII clonal mutants and an isogenic WT clonal line. Genomic DNA from clonal WT or mutants were digested and hybridized to whole genome DNA microarrays. Antimonials are still the mainstay of treatment against Leishmaniasis but in the past decade resistance has been a severe threat. We carried out short read next generation sequencing (NGS) and comparative genomic hybridization (CGH) of three independent Leishmania major antimony resistant mutants. Copy number variations were consistently detected in both NGS and CGH where several chromosomal aneuploidies were correlated to antimony resistance. A major attribute of antimony resistance was a novel terminal deletion of variable length (67kb-204kb) of the polyploid chromosome 31 in the three mutants and was experimentally validated. Terminal d

ORGANISM(S): Leishmania major

SUBMITTER: Frederic Raymond 

PROVIDER: E-GEOD-41750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress