Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from HeLa cells treated with Casiopeina Cas-II-gly

ABSTRACT: Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application. Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly. The cervix-uterine cell line HeLa was maintained at 37C in 5% CO2 under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h. HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Karol Baca Lopez

PROVIDER: E-GEOD-41827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Whole genome gene expression analysis reveals casiopeína-induced apoptosis pathways.

Valencia-Cruz Alejandra Idan AI Uribe-Figueroa Laura I LI Galindo-Murillo Rodrigo R Baca-López Karol K Gutiérrez Anllely G AG Vázquez-Aguirre Adriana A Ruiz-Azuara Lena L Hernández-Lemus Enrique E Mejía Carmen C

PloS one 20130131 1

Copper-based chemotherapeutic compounds Casiopeínas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis-in a process mediated by reactive oxygen species-for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions before establi ...[more]

PMID: 23382936

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Project description:CRISPR-Cas immune systems function to defend prokaryotes against potentially harmful mobile genetic elements including viruses and plasmids. The multiple CRISPR-Cas systems (Types I, II, III) each recognize and target destruction of foreign invader nucleic acids via structurally and functionally diverse effector complexes (crRNPs). CRISPR-Cas effector complexes are comprised of CRISPR RNAs (crRNAs) that contain sequences homologous to the invading nucleic acids and Cas proteins specific to each immune system type. We have previously characterized a crRNP in Pyrococcus furiosus (Pfu) that contains Cmr proteins (Type III-B) associated with one of two primary size forms of crRNAs and functions through homology-dependent cleavage of target RNAs. In the current study, we have isolated and characterized two additional native Pfu CRISPR-Cas complexes containing either Csa (Type I-A) or Cst (Type I-G) proteins and distinct profiles of associated crRNAs. For each complex, the Cas proteins were identified by tandem mass spectrometry and immunoblotting and the crRNAs by RNA deep sequencing and Northern blot analysis. The crRNAs associated with both the Csa and Cst complexes originate from each of seven total CRISPR loci and contain identical 5’ ends (8-nt CRISPR RNA repeat-derived 5’ tag sequences) but heterogeneous 3’ ends (containing variable amounts of downstream repeat sequences). These crRNA forms are distinct from Cmr-associated crRNAs, indicating different 3’ end processing pathways following primary cleavage of common pre-crRNAs. We predict that the newly identified Pfu Type I-A (Csa) and Type I-G (Cst)-containing crRNPs, like other previously characterized Type I CRISPR-Cas effector complexes, each function by carrying out crRNA-guided DNA targeting of invading mobile genetic elements. Taken together, our in-depth characterization of the three isolated native complexes provides clear evidence for three compositionally distinct crRNPs containing either Cmr, Csa, or Cst Cas proteins that together make up an impressive arsenal of CRISPR-Cas defense for a single organism. 4 Samples: Protein-associated small RNAs

Dataset Information

Expression data from HeLa cells treated with Casiopeina Cas-II-gly

Publications

Whole genome gene expression analysis reveals casiopeína-induced apoptosis pathways.

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