Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta

ABSTRACT: To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples of ost1-2 and slac1-5 mutants were compared to their wildtype. Total samples analysed are 11: biological independent replicates of: enriched guard cells (slac1-5) vs. enriched guard cells (gl1-1); enriched guard cells (ost1-2) vs. guard cells (ler);

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Susanne Kneitz

PROVIDER: E-GEOD-42470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype. total samples analysed are 35: 4 biolocigal independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); enriched guard cells (slac1-3) vs. enriched guard cells (gl1-1);guard cells (ost1-2) vs. guard cells (ler);low humidity(20%rh) treated enriched guard cells (COL-0) vs. high humidity(80%) treated enriched guard cells (COL0)

2013-06-04 | E-GEOD-38639 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. total samples analysed are 24: 4 biological independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); low humidity (20%rh) treated enriched guard cells (COL-0) vs. high humidity (80%) treated enriched guard cells (COL-0)

2013-01-14 | E-GEOD-41054 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples of ost1-2 and slac1-5 mutants were compared to their wildtype.

2013-11-20 | GSE42470 | GEO

AtMYB60

Project description:Stomatal guard-cells modulate gas exchange between the plant and the atmosphere. Regulation of transcription is emerging as an important mechanism in controlling guard cells activity. The Arabidopsis transcription factor AtMYB60, is specifically expressed in guard cells and controls stomatal movements. Opening of stomatal pores is constitutively reduced in the atmyb60-1 null allele, and water loss during drought is diminished in the mutant compared to wild type. To address the effect of the AtMYB60 disruption on global gene expression, total mRNAs, derived from atmyb60-1 and WT rosette leaves, grown in standard conditions, were hybridized to a cDNA microarray.

2005-01-10 | E-MEXP-225 | biostudies-arrayexpress

bHLH transcription factors facilitating K+ uptake during stomatal opening are repressed by abscisic acid through phosphorylation

Project description:Stomata open in response to light and close following exposure to abscisic acid (ABA). They regulate gas exchange between plants and atmosphere, allowing plants to adapt to changing environmental conditions. ABA binding to receptors initiates a signaling cascade that involves protein phosphorylation. Here we show that ABA induced phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-RESPONSIVE KINASE SUBSTRATES; AKS1, AKS2, AKS3), in Arabidopsis guard cells, and that they facilitated stomatal opening through the transcription of genes encoding inwardly-rectifying K+ channels. aks1aks2-1 double mutant plants showed decreases in light-induced stomatal opening, K+ accumulation in response to light, activity of inwardly-rectifying K+ channels, and transcription of genes encoding major inwardly-rectifying K+ channels. Overexpression of POTASSIUM CHANNEL IN ARABIDOPSIS THALIANA 1 (KAT1), which encodes a major inwardly-rectifying K+ channel in guard cells, rescued the phenotype of aks1aks2-1 plants. AKS1 bound directly to the promoter of KAT1, an interaction that was attenuated after ABA-induced phosphorylation. The ABA agonist pyrabactin induced phosphorylation of AKSs. Our results demonstrate that the AKS family of bHLH transcription factors facilitates stomatal opening through transcription of genes encoding inwardly-rectifying K+ channels, and that ABA suppresses the activity of inwardly-rectifying K+ channel activity by triggering the phosphorylation of these transcription factors. To find the affect of AKS1 and AKS2 transcription factors on gene expression, Arabidopsis guard cell protoplasts from wild type and aks1aks2-1 mutant were compared. Three independent experiments were performed.

2013-06-17 | E-GEOD-46574 | biostudies-arrayexpress

Identification of genes

Project description:Identifying genes that show differential expression by comparing Atlantic salmon fed with different diets to the negative control group (FM). Comparing gene expression of the distal intestine from Atlantic salmon receiving different diets, totally 57 arrays.

2013-05-30 | E-GEOD-44978 | biostudies-arrayexpress

Transcription profile of CD103- vs CD103+ dendritic cells derived from CX3CR1+c-kit+- bone marrow cells (M1993 M2067)

Project description:We hypothesize that under homeostatic as well as inflammatory conditions circulating monocytes and/or their bone marrow-derived progenitors might contribute to the replenishment of CD103+ and CD103- DC in lymphoid and non-lymphoid compartments. To that end, bone marrow cells from CX3CR1+/gfp C57BL/6 mice were sorted as follows: lineage negative (CD3, CD19, NK1.1, Ter119, Ly6G and CD11c) CX3CR1+c-kit+. Sorted cells were further cultured in vitro under the continuous presence of GM-CSF. lin-CX3CR1+c-kit+ bone marrow cells gave rise to CD103+ and CD103- DC in vitro. To test whether CD103 might be a suitable marker that allows the differentiation of functionally distinct DC subsets generated in vitro, CD11c+CD103+ and CD11c+CD103- DC were sorted from day 5 cultures of lin-CX3CR1+c-kit+ cells and analyzed by whole mouse genome microarrays from Agilent technologies. Compared to CD103+ DC, CD103- DC displayed a strong up-regulation of transcripts for genes involved in innate immunity, whereas those involved in costimulation were down-modulated. Our data suggest distinct functional activity of these two DC subsets. Keywords: Transcriptional profiling-Cell type comparison We compared the transcriptional profile of CD11c+CD103+ and CD11c+CD103- dendritic cells by use of Agilent Whole Mouse Genome Microarrays. Two sets of samples were generated independently as real biological replicates. The microarray analysis was performed as a dual-color experiment, including a dye-swap.

2008-12-17 | E-GEOD-10882 | biostudies-arrayexpress

microRNA signature in b-cell lymphomas and control human B cells from fresh tonsils

Project description:147 B-cell lymphomas were studies for miRNA expression miRNA profiling of control human B cell populations Comparison of miRNA expression profiles of naive B cells (CD19+ IgD+ CD27-) from 3 individuals and germinal center B cells (CD10+ CD19+) from 4 individuals 147 B-cell lymphomas were hybridized on a human miRNA one-color platform containing probes for 470 human miRNAs. Each lymphoma type was compared to all set of NHLs. Burkitt Lymphoma was also directly compared to DLBCL miRNA expression signature is different in human naive B cells compared to Germinal Center B cells

2014-04-12 | E-GEOD-29493 | biostudies-arrayexpress

Microarray-based identification of age-dependent differences in gene expression of human dermal fibroblasts

Project description:Gene expression approach to differences in stress response between human skin fibroblasts derived from chronologically young (~20 years) and old (~90 years) subjects. Expression of CDKN2A was most different between fibroblasts from young and old subjects and Human skin fibroblasts were isolated from skin biopsies of 6 young subjects and 6 old subjects and were treated for 3 hours and 3 days with 0.6 uM rotenone. Also there were control samples, not treated but grown for 3 days, making a total of 36 samples. No experimental replicates were included. The expression of CDKN2A was quantified in the same RNA samples by real-time PCR.

2011-04-01 | E-GEOD-28300 | biostudies-arrayexpress

Analysis of mRNA expression differences in the skin of Casp-8F/–K5-Cre and Casp-8F/+K5-Cre mice

Project description:This SuperSeries is composed of the following subset Series: GSE17344: Analysis of spatial mRNA expression differences in the skin of Casp-8F/–K5-Cre or Casp-8F/+K5-Cre mice GSE17345: Analysis of temporal mRNA expression changes in cultured keratinocytes from Casp-8F/–K5-Cre and Casp-8F/+K5-Cre mice Refer to individual Series

2009-07-29 | E-GEOD-17365 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data