High-throughput 3D screening reveals differences in drug sensitivities between culture models of JIMT1 breast cancer cells.

ABSTRACT: The traditional method for studying cancer in vitro is to grow immortalized cancer cells in two-dimensional (2D) monolayers on plastic. However, many cellular features are impaired in these unnatural conditions and big alterations in gene expression in comparison to tumors have been reported. Three-dimensional (3D) cell culture models have become increasingly popular and are suggested to be better models than 2D monolayers due to improved cell-to-cell contacts and structures that resemble in vivo architecture. The aim of this study was to develop a simple high-throughput 3D drug screening method and to compare drug responses in JIMT1 breast cancer cells when grown in 2D, in polyHEMA coated anchorage independent 3D models and in Matrigel on-top 3D cell culture models. We screened 102 compou

ORGANISM(S): Homo sapiens

SUBMITTER: Vesa Hongisto 

PROVIDER: E-GEOD-42529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress