Dataset Information


FRT thyroid cells: 2D monolayers vs. 3D follicles

ABSTRACT: Transcriptional profiling of the Fischer Rat Thyroid (FRT) cells comparing polarizing cells grown as a confluent two-dimensional monolayer (2D culture system) with cells grown in matrigel where they acquire a three-dimensional follicular structure (3D culture system).The goal was to identify regulators of 3D epithelial thyroid polarization and follicle formation. Overall design: Two condition experiment, 3D vs. 2D FRT cell cultures. Biological replicates: 4 for both conditions. A pool of the 2D replicates was compared with each 3D replicate. One dyeswap replicate comparing 2D pool with 3D pool.

INSTRUMENT(S): Agilent-028279 SurePrint G3 Rat GE 8x60K Microarray (Feature Number version)

SUBMITTER: Petrina Koumarianou  

PROVIDER: GSE71259 | GEO | 2016-11-24



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Pax8 controls thyroid follicular polarity through cadherin-16.

Koumarianou Petrina P   Goméz-López Gonzalo G   Santisteban Pilar P  

Journal of cell science 20161025 1

Organization of epithelial cells during follicular lumen formation is crucial for thyroid morphogenesis and function of the thyroid gland; however, the molecular mechanisms underlying this are poorly understood. To investigate this process, we established three-dimensional (3D) epithelial culture model systems using Fischer rat thyroid (FRT) cells or murine primary thyrocytes that developed polarized spherical structures with a central lumen, mimicking thyroid follicles. Using microarray-based d  ...[more]

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