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NK cells in the female reproductive tract (FRT) acquire an effector transcriptional program during menstruation

ABSTRACT: To evaluate the impact of the menstrual cycle on the transcriptional programs of NK cells in the female reproductive tract (FRT), we compared NK cells (Live lymphocytes, CD45.2+, DX5+, NK1.1+, Ly6G-, CD3-, CD19-) from the spleen and FRT of female C57BL/6J mice either treated with medroxy-progesterone acetate (MPA) or on day 8 of the pseudopregnancy / menstruation protocol. Prior to euthanasia, mice were injected intravenously with anti-CD45 antibody to discern between tissue and circulating NK cells in the FRT, with only tissue-resident cells sorted for analysis. Sorted splenic NK cells were not separated based on CD45 staining. The results indicate that FRT NK cells isolated on day 8 of the pseudopregnancy / menstruation protocol express genes associated with NK cell effector responses compared to FRT NK cells in mice treated with MPA and compared to splenic NK cells in both models.

ORGANISM(S): Mus musculus

PROVIDER: GSE297609 | GEO | 2025/05/30

REPOSITORIES: GEO

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NK cells in the female reproductive tract (FRT) acquire an effector transcriptional program during menstruation

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