GLA and profiling of proteasome, Spt23 and Mga2

ABSTRACT: The proteasome can regulate transcription through proteolytic processing of transcription factors and via gene locus binding, but few targets of proteasomal regulation have been identified. Using genome-wide location analysis and transcriptional profiling in Saccharomyces cerevisiae, we have established which genes are bound and regulated by the proteasome, and by Spt23 and Mga2, transcription factors activated by the proteasome. We observed proteasome association with gene sets that are highly transcribed, controlled by the mating-type loci, and involved in lipid metabolism. At ribosomal protein genes, proteasome and RNA polymerase II binding was enriched in a proteasome mutant, indicating a role for the proteasome in dissociating elongation complexes. The genomic occupancies of Spt2

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Pamela Silver 

PROVIDER: E-GEOD-4272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress