Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

TDP-43 Neurotoxicity in late D. melanogaster pupae

ABSTRACT: The aim of this study is to understand the mechanisms of TDP-43 neurotoxicity. Here, we perform a RNA-Seq analysis in TDP-43 gain-of-fucntion (GOF) , TDP-43 loss-of-function and wild-type late pupae heads (73-90 hours APF) and perform TDP-43 GOF vs wild type and TDP-43 LOF vs wild-type differential expression analysis to show that both mechanisms presents defects in ecdysone receptor (ECR)-dependeint transcriptional program switching, and strongly deregulate expression from the neuronal microtubule associated protien Map205. RNA-seq was performed in two wild-type D.melanogaster biological replicates (Canton S, w1118 ), four biological replicates for TDP-43 (LOF) with two distinct genotypes (dTDP-43Δ142/Df(2R)106,dTDP-43Δ23/Δ142 ) and two TDP-43 GOF biological replicates (act5c>dTDP-43 ).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Bart Dermaut 

PROVIDER: E-GEOD-42844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

TDP-43 proteinopathy is strongly implicated in the pathogenesis of amyotrophic lateral sclerosis and related neurodegenerative disorders. Whether TDP-43 neurotoxicity is caused by a novel toxic gain-of-function mechanism of the aggregates or by a loss of its normal function is unknown. We increased and decreased expression of TDP-43 (dTDP-43) in Drosophila. Although upregulation of dTDP-43 induced neuronal ubiquitin and dTDP-43-positive inclusions, both up- and downregulated dTDP-43 resulted in  ...[more]

Similar Datasets

Unknown TDP-43 Neurotoxicity in late D. melanogaster pupae
2013-01-22 | GSE42844 | GEO
Transcriptomics Identification of KIL1 targets via RNA-Seq in Zea mays
2022-08-16 | E-MTAB-10850 | biostudies-arrayexpress
Unknown Transcriptomic characterization of in vitro human iPSC-derived motor neuron systems for the exploration of cell autonomous mechanisms and modifiers of TDP-43 perturbation associated neurotoxicity
2024-03-23 | GSE261875 | GEO
Unknown TDP-43 modulates translation of specific mRNAs linked to neurodegenerative disease
2018-10-08 | GSE111775 | GEO
Unknown The protective effect of edaravone on TDP-43 plus oxidative stress-induced neurotoxicity in neuronal cells: Analysis of its neuroprotective mechanisms using RNA sequencing
2022-07-11 | GSE207578 | GEO
Proteomics Identify the specific binding partners of cytosolic TDP-43 WT and TDP-43ΔCR
2022-05-20 | PXD027494 | Pride
Unknown Knockdown of transactive response DNA-binding protein TDP-43 downregulates histone deacetylase 6
2009-10-20 | GSE18632 | GEO
Transcriptomics Decrease of Parkin and CD36 expression and body weight correlate with an increase of non-esterified fatty acids in a mouse model for TDP-43 pathology
2014-05-21 | E-GEOD-39585 | biostudies-arrayexpress
Transcriptomics Expression Profiling of Caenorabditis elegans tdp-1 loss-of-function mutant
2012-02-21 | E-GEOD-34113 | biostudies-arrayexpress
Unknown Frontal cortex transcriptomic analysis of a TDP-43 Q331K knock-in mouse [5month]
2018-03-08 | GSE99353 | GEO