TP63 regulation of epithelial to mesenchymal transition in an experimental prostate cell model

ABSTRACT: p63 is a transcription factor central for epithelial homeostasis and development. In our model of epithelial to mesenchymal transition (EMT) in a human prostate cell culture model, p63 was one of the most down-regulated transcription factors during EMT. We therefore investigated the role of p63 in EMT by a gain and loss of function approach. Over-expression of the predominant epithelial isoform DNp63a in mesenchymal EPT1B8 cells led to gain of several epithelial characteristics without resulting in a complete mesenchymal to epithelial transition (MET). This was corroborated by a reciprocal effect when p63 was knocked down in epithelial EP156T cells. Global gene expression analyses found that DNp63a induced gene modules involving cell adhesion genes in mesenchymal like cells. Genome-wide an

ORGANISM(S): Homo sapiens

SUBMITTER: jie liu 

PROVIDER: E-GEOD-43111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress