Functional centromere lost centromeric specific sequences but gain new sequences from nearby chromosomal arm.

ABSTRACT: We identified a newly formed dicentric chromosome (sDic-15) in maize from intrachromosomal recombination and BFB cycles, in which only one centromere is active. The centromeres lost CentC sequences and dramatic reduced the CRM sequences, but when the molecular features of functional centromeres such as CENH3 was examined, they were present. Immunolocalization analysis of phosphorylation of H3T3, H3ser-10 and H2A levels on this new centromere shows a pattern typical of a functional centromere. Meiotic analysis revealed that this dicentric chromosome is table and transmit very well. To examine the new sequences associated with CENH3 in this centromere, chromatin immunoprecipitation (ChIP) was carried out with anti-CENH3 antibodies and material from young seedlings with or without dicentric c

ORGANISM(S): Zea mays

SUBMITTER: Pang Junling 

PROVIDER: E-GEOD-43140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress