Alternate exon switching establishes a tissue-specific transcription factor to mediate temporal activation of gene expression during differentiation

ABSTRACT: Although splicing occurs in most multi-exon genes, the generation of distinct isoforms through the alternate use of mutually exclusive exons is less prevalent. As exon-switching events have the potential to give rise to isoforms with different cellular functions, we have explored the role of the muscle-specific (Mef2Da2) and ubiquitously expressed (Mef2Da1) isoforms of the transcription factor Mef2D in myogenesis. Here we show that both isoforms of Mef2D bind a largely overlapping subset of genomic loci, yet only the muscle-specific Mef2Da2 isoform can activate the late myogenic gene expression program. This differential ability to activate transcription is modulated by PKA signaling where Mef2Da1 is efficiently phosphorylated by the kinase to enhance its association with repressive HDAC-d

ORGANISM(S): Mus musculus

SUBMITTER: Zizhen yao 

PROVIDER: E-GEOD-43223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress