Transcription profiling of cultured rat cardiomyocytes grown with eicosapentaenoic acid or docosahexaenoic acid supplementation in the culture media

ABSTRACT: Despite the recognized protective effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in cardiovascular diseases, and the demonstration of the control of gene expression by polyunsaturated fatty acids (PUFAs), the effects of these n-3 fatty acids on the whole genoma has never been investigated in cardiac cells. Using rat arrays, the effects of Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) supplementation on the global gene expression profile were evaluated in cultured neonatal rat cardiomyocytes. Experiment Overall Design: Primary cardiomyocyte cultures were obtained from the ventricles of newborn Wistar rats and grown in HAM F10 plus 10% fetal calf serum and 10% horse serum medium (controls), or in the same medium supplemented with 60 M Eicosapentaenoic

ORGANISM(S): Rattus norvegicus

SUBMITTER: Alessandra Bordoni 

PROVIDER: E-GEOD-4327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress