Defining the microglia transcriptome during disease progression in ALS transgenic mice

ABSTRACT: Purpose: We purified spinal cord microglia utilizing percoll gradients and magnetic beads, followed by transcriptome profiling (RNA-seq) to define microglia expression profiles against other neural, immune cell-types. We next observed how the microglial transcriptomes change during activation in the SOD1-G93A mouse model of motor neuron degeneration at 3 time points. We also compared these profiles with that induced by LPS injection. Results and conclusions: ALS microglia were found to differ substantially from those activated by LPS and from M1/M2 macrophages by comparison with published datasets. These ALS microglia showing substantial induction of a neurodegeneration-tailored phenotype, with induction of lysosomal, RNA splicing, and Alzheimer's disease pathway genes. Overall they expre

ORGANISM(S): Mus musculus

SUBMITTER: Sean O'Keeffe 

PROVIDER: E-GEOD-43366 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress