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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Asthma

ABSTRACT: Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asthmatic (MMA) and severe asthmatic (SA) patients. Isolated fresh bronchial epithelial cells were placed into trizol for further analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Jadranka Milosevic

PROVIDER: E-GEOD-43696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An airway epithelial iNOS-DUOX2-thyroid peroxidase metabolome drives Th1/Th2 nitrative stress in human severe asthma.

Voraphani N N Gladwin M T MT Contreras A U AU Kaminski N N Tedrow J R JR Milosevic J J Bleecker E R ER Meyers D A DA Ray A A Ray P P Erzurum S C SC Busse W W WW Zhao J J Trudeau J B JB Wenzel S E SE

Mucosal immunology 20140212 5

Severe refractory asthma is associated with enhanced nitrative stress. To determine the mechanisms for high nitrative stress in human severe asthma (SA), 3-nitrotyrosine (3NT) was compared with Th1 and Th2 cytokine expression. In SA, high 3NT levels were associated with high interferon (IFN)-γ and low interleukin (IL)-13 expression, both of which have been reported to increase inducible nitric oxide synthase (iNOS) in human airway epithelial cells (HAECs). We found that IL-13 and IFN-γ synergist ...[more]

PMID: 24518246

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