Pervasive and dynamic protein binding sites of the mRNA transcriptome in Saccharomyces cerevisiae

ABSTRACT: Protein-RNA interactions are integral components of nearly every aspect of biology including regulation of gene expression, assembly of cellular architectures, and pathogenesis of human diseases. However, studies in the past few decades have only uncovered a small fraction of the vast landscape of the protein-RNA interactome in any organism, and even less is known about the dynamics of protein-RNA interactions under changing developmental and environmental conditions. Here, we describe the gPAR-CLIP (global photoactivatable-ribonucleoside-enhanced crosslinking and immunopurification) approach for capturing regions of the transcriptome bound by RNA-binding proteins (RBPs) in budding yeast. We report over 13,000 RBP crosslinking sites in untranslated regions (UTR) covering 72% of protein-cod

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Mallory Freeberg 

PROVIDER: E-GEOD-43747 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress