Response of methicillin-resistant Staphylococcus aureus USA300 to a subinhibitory concentration of thioridazine - alone and in combination with dicloxacillin

ABSTRACT: Subinhibitory concentrations of the neuroleptic drug thioridazine (TDZ) are well-known to enhance the killing of methicillin-resistant S. aureus (MRSA) by β-lactam antibiotics, however, the mechanism underlying the synergy between TDZ and β-lactams is not fully understood. In the present study we have examined the effect of a subinhibitory concentration of TDZ on antimicrobial resistance, the global transcriptome, and the cell wall composition of MRSA USA300. We show that TDZ is able to sensitize the bacteria to several classes of antimicrobials targeting the late stages of peptidoglycan synthesis. Furthermore, our microarray analysis demonstrates that TDZ modulates the expression of genes encoding membrane and surface proteins, transporters, and enzymes involved in amino acid biosynthe

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300_FPR3757

SUBMITTER: Mette Thorsing 

PROVIDER: E-GEOD-43759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress