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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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TMEM88 knockdown during human embryonic stem cell cardiac differentiation

ABSTRACT: TMEM88 is indispensable for heart development and acts in the pre-cardiac mesoderm to specify lineage commitment of the cardiovascular progenitor cell through inhibition of Wnt signaling. 2 different lentiviruses expression shRNA targeting different domains of the TMEM88 locus were transduced into undifferentiated hES cells. Cells were puromycin selected then differentiated along the cardiac lineage. Total RNA was taken at day 5 of differentiation when the cardiovascular progenitor cell arises. 1 sample for each shRNA and 2 samples for control shRNA were analyzed by array

ORGANISM(S): Homo sapiens

SUBMITTER: Nathan Palpant

PROVIDER: E-GEOD-43805 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transmembrane protein 88: a Wnt regulatory protein that specifies cardiomyocyte development.

Palpant Nathan J NJ Pabon Lil L Rabinowitz Jeremy S JS Hadland Brandon K BK Stoick-Cooper Cristi L CL Paige Sharon L SL Bernstein Irwin D ID Moon Randall T RT Murry Charles E CE

Development (Cambridge, England) 20130807 18

Genetic regulation of the cell fate transition from lateral plate mesoderm to the specification of cardiomyocytes requires suppression of Wnt/β-catenin signaling, but the mechanism for this is not well understood. By analyzing gene expression and chromatin dynamics during directed differentiation of human embryonic stem cells (hESCs), we identified a suppressor of Wnt/β-catenin signaling, transmembrane protein 88 (TMEM88), as a potential regulator of cardiovascular progenitor cell (CVP) specific ...[more]

PMID: 23924634

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TMEM88 knockdown during human embryonic stem cell cardiac differentiation

Project description:TMEM88 is indispensable for heart development and acts in the pre-cardiac mesoderm to specify lineage commitment of the cardiovascular progenitor cell through inhibition of Wnt signaling.

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Human induced pluripotent stem cells: diploid, chr7q deletion, spontaneous chr7 correction

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2015-02-16 | E-GEOD-65215 | biostudies-arrayexpress

Sox7 regulates lineage decisions in cardiovascular progenitor cells

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2019-07-06 | GSE133899 | GEO

Sox7 regulates lineage decisions in cardiovascular progenitor cells

Project description:Sox7 regulates lineage decisions in cardiovascular progenitor cells

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Notch Activation Commits Mesoderm Cells to the Cardiac Lineage [cardiomyocytes]

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